HPLC column thermostat is a temperature control instrument. It is used to control the temperature of the liquid chromatography column in the analysis of high performance liquid chromatography. Can accurately and stably control the stability of liquid chromatography columns.
It is beneficial for improving the sensitivity of the liquid chromatography column, improving the resolution of spectral peaks, accelerating separation speed, shortening analysis time, and ensuring analysis results. Accuracy and reproducibility. For high-performance liquid chromatography, an increase in column temperature can accelerate the separation process.
The methods of separation controlled by liquid chromatography include distribution, adsorption, ion exchange, and size exclusion. These equilibria depend on temperature, and the column temperature is maintained through a column temperature box.It can achieve good reproducibility in sample separation, selectivity, and retention time. Additionally, as the temperature increases, the equilibrium between the target component and the stationary phase becomes faster.It result in an increase in column efficiency and improved separation. In addition, the column temperature box helps to stabilize electrical signals, reduce signal fluctuations. And also helps to reduce the viscosity of the liquid, thereby reducing column pressure. The following are common precautions for the high efficiency liquid phase column temperature box module